| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Leptospira borgpetersenii | GCAGGTTGCGGACAATTCC | CGTCGATGATCGCACAAGC | 59.79 | 59.73 | 266 | 120 |
100.00%
|
100.00%
|
| Primer 2 | Leptospira borgpetersenii | CGATCCCGATCCGAGAACTG | TGCCAGGAGAACTTCGACC | 60.04 | 59.33 | 170 | 120 |
100.00%
|
100.00%
|
| Primer 3 | Leptospira borgpetersenii | TCGAAGAACCGAGGCAAGG | ACCTTCTCCCGACTTCAGG | 59.71 | 58.33 | 172 | 119 |
100.00%
|
100.00%
|
| Primer 4 | Leptospira borgpetersenii | TCCGGTGACTTCCCAAAGG | TTTGTCCGTGAGCGAGAGG | 59.24 | 59.71 | 251 | 112 |
100.00%
|
100.00%
|
| Primer 5 | Leptospira borgpetersenii | GCATAACGCGTACCGAAGTC | ATCACTTCCAGCTGTCGGC | 59.43 | 60.08 | 227 | 99 |
100.00%
|
100.00%
|
| Primer 6 | Leptospira borgpetersenii | GCATAACGCGTACCGAAGT | ATCACTTCCAGCTGTCGGC | 58.34 | 60.08 | 227 | 99 |
100.00%
|
100.00%
|
| Primer 7 | Leptospira borgpetersenii | TCCAACTGCGCAACGAGT | TGCGATACTTCCTTCTCCACC | 59.89 | 59.52 | 177 | 99 |
100.00%
|
100.00%
|
| Primer 8 | Leptospira borgpetersenii | TACGGGTTCCACTGAGGTG | TCACTTACGAAGTCCGAGAGT | 58.64 | 58.22 | 208 | 99 |
100.00%
|
100.00%
|
| Primer 9 | Leptospira borgpetersenii | GGGAGATACGGGTTCCACTG | TCACTTACGAAGTCCGAGAGT | 59.54 | 58.22 | 214 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Leptospira borgpetersenii | ATACGGGTTCCACTGAGGTG | TCACTTACGAAGTCCGAGAGT | 59.10 | 58.22 | 209 | 99 |
100.00%
|
100.00%
|